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Comparison of results obtained with Amplicor HIV-1 DNA PCR test version 1.5 using 100 versus 500 microliters of whole blood.

Piwowar-Manning E, Lugalia L, Kafufu B, Jackson JB

Department of Pathology, 600 North Wolfe St., Johns Hopkins Medical Institutions, Baltimore, MD 21287, USA.

The Amplicor HIV-1 DNA PCR assay (Roche Diagnostics, Branchburg, NJ) requires 500 microl of whole blood for a diagnosis of human immunodeficiency virus type 1 (HIV-1) infection, and this amount is often difficult to obtain from infants. A comparison was performed using 100 and 500 microl of whole blood from infants less than 18 months of age. The concordance rate for HIV DNA PCR-negative and -positive samples was 100% for the two different volumes.

Published 10 March 2008 in J Clin Microbiol, 46(3): 1104-5.
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